WebCy3 (Cyanine-3) is a fluorescent compound with an excitation peak at 555 nm and an emission peak at 569 nm. It can be excited using a 561 nm laser paired with a 585/20 nm bandpass filter, a configuration that can be found, for example, in the BD Special Order LSRFortessa™ Cell Analyzer. WebCy3 Acid (also sold under Sulfo-Cyanine3 Acid) is a bright, water-soluble, and pH insensitive> from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the …
Recommended dye combinations for multiplex qPCR - Clark …
WebCy3 dye. Fluorescence SpectraViewer. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC … WebCy3 Dye If the Cy3 dye is sufficiently close (2–9nm) to the Cy5 (acceptor) dye, energy transfer can occur resulting in a mixture of emitted light coming from the donor and acceptor dyes. From: Methods in Enzymology, 2009 Related terms: Protein Oligonucleotide Quantum Dot Nanoparticle Cy5 Dye Fluorescence Resonance Energy Transfer MicroRNA funny shocked pictures
Protein Environment and DNA Orientation Affect Protein-Induced Cy3 ...
WebDec 11, 2013 · Dye free acids (CypHer5, Cy3.29 or Cy5.29, 3, 1 and 2 respectively) were derivatized to make -NBoc conjugates (5, 6, 7) using ethylenediamine-NBoc in organic solvents and purified by preparative HPLC. ... The Cy3 reference signal was used to quantify the degree of nanoemulsion uptake during cell labeling. Cellular auto … WebFigure 5. Molecular structures of the Cy3 and Cy5 cyanine dye phosphoramidites used in this study. After the end of the synthesis and the chemical deprotection step, the dyes … Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … git diff between branches name only